amani shalamzari

The aim of this study was to assess The effects of aerobic training on Nf-kB, Lin28B and let-7a microRNA expressions and levels of tumor tissue IL-6 in mice with breast cancer. Twenty BALB/c c mice (4-5 weeks,17g mass) were cancerous by injection of estrogen-dependent receptor breast cancer cells MC4-L2 and divided into two groups: tumor-training(TT) and tumor-control(TC) group. Then TT group completed aerobic training for 6 weeks, 5 days per week (14-18 m/min). 48 hours after the last exercise subjects were sacrificed. Tissue sampling were collected and stored in -70ᵒ. Nf-kB, Lin28B and let-7a microRNA expressions were accounted with Real time-PCR and IL-6 levels was accounted with ELISA Kit. Repeated measures and independent t tests were used to assess tumor size and IL-6, respectively. Statistical analysis of Nf-kB, Lin28B and let-7a were conducted by REST software. Tumor size, Nf-kB, Lin28B and IL-6 levels were significantly decreased in TT group compare with TC group (p<0.05). microRNA let-7a was significantly increased in TT against control group respectively (p=0.000). In breast cancer, a positive feedback loop consisting of Nf-kB, Lin28B, microRNA let-7a and IL-6 is activated. It seems that regarding to the depressing effects of aerobic training on this regulatory circuit in mice with breast cancer, This type of training can be used as adjuvant therapy in conjunction with other therapies for estrogen receptor dependent breast cancer.

Obesity is a state characterized by a low-grade inflammation that leads to insulin resistance. The aim of the present study was to assess serum interleukin-18 (IL-18), interleukin-6 (IL-6), C-reactive protein (CRP) and Homeostasis Model Assessment of Insulin Resistance (HOMA-IR) in response to circuit resistance exercise in obese and normal-weight subjects with different levels of physical activity. Thirty-two healthy male students participated in the present study. Subjects were divided into 4 groups according to their BMI and level of physical activity: active obese (n=8), active non-obese (n=8), inactive obese (n=8), and inactive non-obese (n=8). To determine serum IL-6, IL-18, CRP, glucose and insulin concentrations, fasting and post-exercise blood samples were obtained. Subjects performed a bout of circuit resistance exercise in 2 sets with 10 repetitions at 60% of 1RM. Obese subjects comparing non-obese ones showed significant increase in IL-6 and significant decrease in IL-18 concentrations in response to exercise (p<0.05). There was no significant difference between active normal and inactive normal subjects in response to exercise. Also, there were not significant differences in four groups in response to exercise. The significant decrease in IL-18 concentration in the obese group comparing normal group in response to exercise was probably due to anti-inflammatory effects of exercise. Also, recommending this kind of exercise for obese persons with low level of physical activity can improve insulin resistance.