mohammad ghaderzadeh

On the contrary, the grain-fed steers received a grain-based regime that served as an efficient source of high-digestible energy. Rumen may function differently in the grass- and grain-fed regimes. Therefore, grain-fed cattle suffer stronger metabolic stress than pasture-fed steers; and they tend to easily have metabolic and infectious diseases. In this study to gain insights into transcriptional regulation in spleen and rumen tissues under two different regimes include grass and grain, the first high expression genes in two different conditions were identified by RNA-seq data analyses. Promoter analysis was performed using a bioinformatics database of Genomatix. Moreover, in this study to the visualization of regulatory networks containing transcription factors and that regulate the genes in the rumen and spleen, were used Cytoscape software. Then, promoter analysis leads to the identification of 31 novel transcription factor activating in the rumen and 10 novel transcription factors candidates in the spleen in cow fed with grass and grains. Results revealed that 10 genes with the highest expression were identified in both rumen and spleen. According to the analysis of the results in the David Database, the processes: reduction of oxidation, regulation of cell proliferation, ion transport, epididymal development ,and ectoderm development were evaluated as significant. The results in this study provide valuable insights into molecular mechanisms in spleen and rumen tissues under two different regimes include grass and grain.

Data used in this study related to growth traits in Lori sheep that were collected during 2001 - 2010 by Research Center for Agriculture and Natural Resources of Lorestan province. Growth traits were consist of different ags (brithy, 3 month, 6 month and 9 month). Avarage weight Lori sheep was in birth weight 3.49 kg and in 9 month weight 29.79 kg. The SAS statistical software was used to determine the effect of environmental factors and WOMBAT software was applied to estimate genetic parameters with 6 different models via animal model analysis one trait REML parameters. Environmental factors like birth year, sex, type of birth and age of mother on lambing time had significant effect on all traits (p<0.0001). Type of birth and sex had significant effect on all traits except the 9 month age (p<0.0001). The direct heritability using best chosen model for birth weight, weaning weight, 6 months weight, 9 months weight were respectively obtained as follows: 0.34, 0.01, 0.16 and 0.1 and mathernal heritability for these traits were estimated 0.16, 0.03, 0.05 and 0.06, respectively.

In this study to gain insights into transcriptional regulation Lactogenesis in mammary tissue, at the first high expression genes in lactogenesis in three different condition were identified by RNA-Seq data analysis. This three conditions including comparison of the dairy cow mammary tissue in 35 days before and 3 days after birth, dairy cow mammary tissue in 7 days before and 3 days after birth and dairy cow mammary tissue in 7 and 35 days before birth. To investigate the biological processes of significant high expression genes in three different condition mentioned above, the DAVID software was used. The only significant terms (P

Infectious bronchitis is one of the widespread and highly infectious viral disease of poultry. In this study to gain insights into transcriptional regulation of infection bronchitis, at the first high expression genes in infection bronchtis in four different conditions were identified by RNA-Seq data analysis. In this study to gain insights into transcriptional regulation of infection bronchitis, at the first high expression genes in infection bronchitis were identified by RNA-Seq data analysis in four different conditions including comparison of the spleen chickens with bronchitis versus healthy with low serum concentration of mannose-binding lectin at the age of one and three weeks, comparison of the spleen chickens with bronchitis versus healthy with high serum concentration of mannose-binding lectin at the age of one and three weeks. Then promoter regions of these genes were extracted to identify candidate transcription factors involved in this mechanisms. Promoter analysis by using Genomatix software lead to identify of 61 novel transcription factors candidates activating in infection bronchitis. In this study in order to visualization of regulatory networks containing transcription factors and that regulate the genes, the were used Cytoscape software. According to the results, 61 new candidates transcription factor introduced in this study has good potential for more inestigation in the laboratory level and can provide new information to better understanding of regulatory networks in infectious bronchitis in poultry.

Inroduction: Inbreeding is defined as the probability that two alleles at any locus are identical by descent and occur when related individuals are mated to each other. The initial consequence of inbreeding is inbreeding depression reducing the performance of growth, production, health, fertility, and survival traits. This concern has become more serious in animal breeding nowadays, in which selection responses are maximized using animal model best linear unbiased predictors (BLUP) of breeding value. The use of these breeding values alone may result in more closely related selection candidates preferred for selection, with increased levels of inbreeding since they share most of their familial information. The unavoidable mating of related animals in closed populations leads to accumulation of inbreeding and decreased genetic diversity. Inbreeding has deleterious effect on additive genetic variance as well as on phenotypic values. Heterozygosity and allelic diversities can be lost from small, closed, selected populations at a rapid rate. The rates of inbreeding must be limited to maintain diversity at an acceptable level so that genetic variation will ensure that future animals can respond to changes in environment. Inbreeding depression has been found in a large proportion of species examined. Lori sheep breed is mainly raised in Lorestan Province in Iran. This study was conducted to identify pedigrees and inbreeding depression on growth traits in Lori sheep population from 2001 to 2010. The rate of inbreeding needs to be limited to maintain diversity at an acceptable level so that genetic variation will ensure that future animals can respond to changes in the environment and to selection. Without genetic variation, animals cannot adapt to these changes. For this study pedigree information and body weight at different ages (birth weight, weaning weight, 6-month weight, and 9-month weight) of 6440 lambs from 273 rams and 1955 ewes during the years 2001 to 2010 from Lorestan Agricultural and Natural Resources Research Center were used. The pedigree corrections were done by the Excel program and the estimation of inbreeding coefficients was done by pedigree software. To study the pedigree, Pedigree, program, or estimate inbreeding coefficient, CFC program, and for calculate the amount of inbreeding citizenship traits, wombat software were used. In total population the number of inbred animals were 2126 (33%) of the herd. The average total population inbreeding coefficient and average inbreeding coefficient inbred of population were 0.69% and 2.24%, respectively. Annual changes in inbreeding coefficient was 0.21, which was statistically significant (p