r. shekarriz

Considering the anti-cancer effects of statins on colon cancer previously reported in epidemiological studies and uncertainty about the mechanisms of action, this study was performed to investigate the effect of statins in combination with chemotherapy on colon cancer cell lines.

The present study, which is a laboratory study, was performed on HT-29 cell line as colon cancer cell line and normal HFF cell line. After cell culture, atorvastatin at doses of 15 and 30 μM, rosuvastatin at doses of 30 and 60 μM, simvastatin at doses of 10 and 20 μM and 5-fluorouracil (5-FU) at 20 μg/ml were used for interventions in this study. Interventions were performed alone and in combination. Finally, MTT test was performed to evaluate cell viability.

Mean cell viability in simvastatin (20 μM) and simvastatin (20 μM)+5-FU (20 μg/ml) on colorectal cancer cell lines was 58.46±7.58 and 55.73±15.33 (p=0.999), respectively, in rosuvastatin (30 μM) and rosuvastatin (30 μM)+5-FU (20 μg/ml) was 91.63±10.3 and 56.2±10.27 (p=0.026), respectively, and in atorvastatin (30 μM) and atorvastatin (30 μM)+5-FU (20 μg/ml) was 82.3±26.9 and 52.16±9.49 (p=0.24), respectively.

The evaluation of cell proliferation showed that the addition of rosuvastatin to standard chemotherapy, unlike atorvastatin and simvastatin, had an increasing effect on cytotoxicity.

Correct and timely detection of the outbreaks of diseases with a short incubation period is of great importance in the health system. The aim of this study was to determine the detection of dysentery outbreaks using the cumulative sum method.

This time series study was conducted using the data of the National Surveillance System between 2014 and 2017. The outbreak alert threshold of each season and province was determined separately using the average of three years (1393 to 1395) in the same season and province. The dysentery outbreak in each season was exclusively predicted for Isfahan, Khuzestan, and Hamadan provinces in 2017 using the CUSUM method.

In Isfahan Province, the outbreak alert was higher in the spring and summer and lower in the autumn and winter using the current method compared to the CUSUM method. For Khuzestan Province, the current outbreak alert was significantly higher in all seasons compared to the CUSUM method, while the current outbreak alert was lower than the alert predicted by the CUSUM method in Hamadan Province in all the seasons.

Compared to constant threshold-based methods, using the CUSUM method seems to be a better way for reporting outbreaks, especially in areas with a high incidence.