جستجوی مقالات مرتبط با کلیدواژه « total alkaloid » در نشریات گروه « زیست شناسی »

Medicinal plants are the most exclusive source of life saving drugs for majority of the world’s population. Plant tissue cultures can be established routinely under sterile conditions from explants, such as plant leaves, stems, roots, etc. Both for multiplication and extraction of secondary metabolites. Solanaceous plants, such as genus Hyoscyamus, are regarded as rich sources of tropane alkaloids. Present study, H.arachnoideus Pojark, were investigated. Three types of explants (leaf, root) derived from seedlings, were cultured on Murashige and Skoog’s medium supplemented with 3% sucrose, 0.75% (w/v) agar. We investigated the effect of different concentrations of Ca2+, NO-3 on Murashige and Skoog’s medium supplemented with 3% sucrose and 0.75% (w/v) agar in two explant types (leaf and root) of H.arachnoideus with four concentration levels of auxin naphthalene acetic acid(0, 0.5, 1, 2 mg/l), in compare with Murashige and Skoog’s medium supplemented with 3% sucrose, 0.75% (w/v) agar as control, In order to determine callus biomass and total alkaloid content. Our findings showed that, increased concentrations of Ca2+ was found to have positive effect on callus biomass and total alkaloid content. NO-3 had different effects on callus biomass and alkaloid production, but increased concentrations of NO-3 could enhance callus biomass and decrease alkaloid content, in some cases.

Therapeutic effects of Hyoscyamus genus are attributed to its obtained different tropan alkaloids. Regarding these rare medicinal herbal species, tissue culture is of almost importance, as, it is possible to obtain optimal volumes of suitable secondary metabolite- producing-tissues with alteration in their culture media. Present study, H.arachnoideus Pojark, was investigated. Three types of explants (leaf, root, and hypocotyls) derived from seedlings, were cultured on Murashige and Skoog’s medium supplemented with 3% sucrose, 0.75% (w/v) agar. We investigated the effect of two types of carbon sources (sucrose and monitol) on Murashige and Skoog’s medium supplemented with 3% sucrose and 0.75% (w/v) agar in two explants types ( leaf and root) of H.arachnoideus with four concentration levels of auxin naphthalene acetic acid(0, 0.5, 1, 2 mg/l), in compare with Murashige and Skoog’s medium supplemented with 3% sucrose, 0.75% (w/v) agar as control, In order to determine callus biomass and total alkaloid content. Our findings revealed the bilateral role of sucrose both as nutrient source and osmotic regulator factor, and also showed the positive effect of sucrose on callus biomass and total alkaloid content.

The tubers of Bongardia chrysogonum، belonging to to Podophyllaceae، are rich in alkaloids. In this study، total alkaloid content of the tubers of this plant in Naghadeh، Zanjan and Boukan were determined by spectrophotometeric method. In addition، the effects of soil characteristics such as total nitrogen، potassium، pH، soil texture and tubers nitrate levels on the plant total alkaloid content were measured. The results of the analyses indicated significant differences between the total alkaloid content of B. chrysogonum in these three regions. Moreover، the results showed significant differences among nitrates amounts of this plant in these habitats. The highest and lowest nitrate contents belong to Naghadeh and Boukan plants، respectively. The evaluation of soil samples of these three regions indicated that the texture of soil in Naghadeh is clay-loamy، in Zanjan it is silty- clay- loamy and in Boukan it is loamy- sandy. It was also discovered that increase in plant total alkaloid content depends on the increase of sand percentag in soil to some extent. The effect of soil pH on total alkaloid content turned out to be nonsignificant.