جستجوی مقالات مرتبط با کلیدواژه « Glucose-6-phosphate dehydrogenase » در نشریات گروه « پزشکی »

Red blood cell (RBC) storage lesions may dramatically reduce the function of these cells. One of these complications is the gradual increase in oxidative stress, which causes the destruction of RBCs during their storage. This study was designed to investigate the effect of glutathione additive as an antioxidant on the biochemical changes of leukoreduced-RBC (LR-RBC) bags during storage.

The study was experimental. In 2016, it was done in Tehran Blood Transfusion Organization. Ten bags of LR-RBC were collected, processed and stored according to standard procedures. Each unit was divided into two equal parts treated with glutathione or normal saline (control). Sampling of all bags was done during the time points of 3, 14, 21, 35 and 42 days after storage. Used the ELISA method to investigate changes in glutathione and 2,3-diphosphoglycerate (2,3-DPG) and the enzymatic method to measure biochemical parameters such as lactate-dehydrogenase (LDH), lactate concentration and glucose-6-phosphate-dehydrogenase (G6PD) enzyme activity.

The amount of glutathione in the treated group and the control group decreased by 70 % and 60% of the initial value, respectively. With the decrease of glutathione level in the last week of storage, a significant decrease in G6PD activity was observed. The amount of 2,3-DPG decreased to almost zero after 42 days of storage. LDH and lactate increased during storage.

Our results showed that, except for the amount of 2,3-DPG, the rest of the studied parameters were affected by glutathione and glutathione antioxidant was effective in reducing oxidative stress

Despite wide fight against malaria in endemic regions, it is still one of the most serious infectious diseases in some parts of the world. Primakin is effective in treatment and prevention of malaria. However, individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency are at risk for hemolysis and its complications when taking primakin. This study was performed to determine the prevalence of G6PD deficiency in patients suspected of favism in Qaleh Ganj, Kerman, Iran.

This study was conducted in 2020 and blood samples were taken from all patients suspicious of favism attending Shohada Hospital in Qaleh Ganj, southern Iran. The G6PD enzyme activity was evaluated by fluorescent spot test using a commercial kit. Data were analyzed using SPSS V23.

In this study, 235 patients (88 males and 147 females) were examined. Fifty eight people (24.7%) had abnormal levels (deficiency) of G6PD enzyme, 38 (65.6%) of whom had severe deficiency and 20 (34.4%) had partial deficiency. No significant relationship was found between sex and G6PD level (P= 0.96).

The study showed a high prevalence of G6PD deficiency in this region. So, attention should be paid to this issue when prescribing oxidative drugs, especially antimalarial drugs.