جستجوی مقالات مرتبط با کلیدواژه « Root MTA » در نشریات گروه « پزشکی »

This in vitro study compared the cytotoxic effects of three commercially available MTA formulations naming ProRoot MTA (PMTA), Angelus MTA (AMTA), and Root MTA (RMTA), with calcium-enriched mixture (CEM) cement and a new nanohybrid MTA (NMTA) on human dental pulp stem cells (DPSC). Methods and Materials: Four disc-shaped specimens of each material were prepared. After completion of setting, 2 different (neat and 1/2) elutes of the test materials were made. Then in each cavity of a 96-well plate, 3000 cells were seeded and incubated in a humidified incubator with 5% CO2 and 95% air at 37°C for 24 h. After this period, the culture medium of each well was replaced with 200 μL of test material elutes. Plain culture medium was used as the negative control and distilled water as the positive control group. Cell viability was assessed using 2, 5-diphenyl-SH-tetrazelium bromide colorimetric assay, aka Mosmann’s tetrazolium toxicity (MTT) assay, at three time intervals (24, 48, and 72 h after mixing). Data were analyzed using the ANOVA and Tukey’s post hoc test (P=0.05). After 24 h, the viability of cells in neat concentration had no significant differences (P>0.05) except for the NMTA. However, CEM and AMTA, at 1/2 concentration exerted significant proliferative effects on cells. At 48 and 72-h intervals, significant proliferation of DPSCs was seen in all samples, except for the NMTA which exerted toxic effects on cells. All of the three commercial MTAs and CEM cement showed comparative biocompatibility. However, NMTA had cytotoxic effects on DPSCs at all the time intervals.

Root perforations are an undesired complication of endodontic treatment which result in loss of integrity of the root, and adversely affect the prognosis of the treatment. Recently, Iranian mineral trioxide aggregate (Root MTA) has been introduced as an ideal material for perforation repair. In this article a successful repair of strip root perforation of mandibular molar using Root MTA is presented with 15-month follow-up. This case suggests that Root MTA may be a substitute material for the treatment of strip perforation; however, more clinical studies with larger sample size and longer follow-ups are needed.

Introduction
The materials used in sealing furcating perforation can have considerable effects on controlling the ensuing inflammation and periodontal repair. The objective of the present study was to carry out a histological comparison between the effects of pro-root, cold ceramic, glass-ionomer cement, and root MTA on the healing of periodontal tissues after furcal perforation in dog''s teeth.
Methods and Materials
One-hundred premolar teeth of one-year old dogs were used in this experimental/animal study. After anesthetizing the dogs and the premolar teeth, the access cavities were prepared at the occlusal level and the root canals were instrumented and filled with gutta percha and AH26 sealer, using the step-back technique. Furcations were perforated to a size of 3×3 mm2, using long burs. These areas were then randomly filled with aforementioned four test materials (a total number of 84 premolar teeth) while the access cavities were filled with amalgam. The remaining 16 teeth were selected to serve as positive and negative controls. Biopsy samples were taken from the perforated areas at 1, 2, and 3-month intervals and were transferred to laboratory for pathological examination. The results were statistically analyzed, using the Kruskal-Wallis and Mann Whitney tests.
Results
The statistical analysis revealed that under similar conditions, periodontal tissues surrounding Pro-root, show less inflammatory response than the other three materials. However, no significant differences were observed among the four studied materials during 1 and 2months as evidenced by the biopsy samples (P>0.05). For longer period (three month), however, samples surrounding cold ceramic and Root MTA showed decreasing inflammatory responses.

Discussion
From the findings of the present study, it may be concluded that although tissues adjacent to Pro-root showed less inflammatory response than other three test materials, all of them (Pro-root, Glass-ionomer cement, cold ceramic, and Root MTA) may be considered to be suitable materials for sealing furcal perforation providing. They receive approval by other tests including micro leakage, cytotoxicity, tissue analysis, and etc.