The Effect of Metformin on Neutrophil Recruitment in Carrageenan-Induced Peritonitis in Mice

An acute inflammation is immediate and rudimental responses to a destructive factor and neutrophils have a basic role in immunoinflammatory responses. It has been shown that AMPK (5 Amp Activated protein Kinase) exists in neutrophils and its activation reduces the activity of neutrophils and can be a suitable pharmacological target in curing the acute inflammatory procedures. We aimed to study the effect of metformin a pharmacological activator of AMPK on leukocytes accumulation and neutrophil activity in peritonits induced by carrageenan in mice. Male Albino mice (25-30g) received orally 25,50 and 100 mg/kg metformin one hour prior to induction of peritonites by intraperitoneal injection of carrageenan 1% (0.2 ml). Control animal recieved saline four hours after peritonites induction. Leukocytes were counted and neutrophils differentiation and myeloperoxidase activity were determined in the exudate. The results of this study demonstrated that acute treatment of mice with single dose (50mg/kg) of Metformin significantly reduced leukocytes accumulation and neutrophil activity. Metformin noticabaly reduces the acute activation of neutrophiles of Ampk pathwaye by metformin is considered as a possible mechanism for this anit-inflammatory effect.