Tomato transformation by recombinant vector containing PR1, chitinase and glucanase genes

Tomato is one of the most important crops in the world which is vastly cultivated everywhere. This crop bears a great loss every year due to the presence of pathogenic fungi such as Fusarium species. In present study in order to strengthen defense mechanism of tomato, we transferred coincidently the genes PR1, chitinase and gluconase into tomato. To do so, first, the gene PR1 was extracted from tobacco genome and then replicated under the control of 35S promoter and Nos terminator in recombinant vector pBI121. In addition, chitinase and gluconase genes were replicated under the control of independent 35S promoter and Nos terminator in vicinity of PR1 gene. It is followed by transformation of tomato explants with PRP-1ChiGlu(-) containing PR1, chitinase and gluconase genes by using recombinant vector pBI121. In this transformation, leaf explants placed on the pre-media were inoculated by Agrobacterium tumefaciens LBA4404 containing aforementioned vector. These explants were transferred first to similar media and then to regeneration media and every seven days were subjected to subculture and after 4 weeks they were transferred to root generation media containing 200 ml/lit Cefotaxime and 25 mg/lit Kanamycin. Molecular analysis of PCR and dot blotting showed that transgenic plants had at least one copy of in question genes on their genome.