Polymorphism Study of 9 and 11 Exons of Leptin Receptor Gene in Mazandaran Native Chickens by PCR-RFLP technique

Leptin is a polypeptide hormone that was identified as the main secretion of adipose tissue. It involved in regulation of feed intake, energy metabolism and fertility. Purpose of this study was to identify allelic polymorphism in Leptin gene receptor. We have used a restriction fragment length polymorphism (RFLP) method. Blood samples were randomly collected from 100 native chickens in Sari fowl breeding station. The DNA extraction was based on salting – out method. The quantity and quality of extracted DNA were examined using spectrophotometric and agarose gel electrophoresis. A strategy employing polymerase chain reaction was used to amplify a 374 bp fragment of 9-11 exon Leptin gene receptor. Digestion of amplicons with HaeIII revealed Leptin gene receptor. On results of band pattern digestion with HaeIII be showed no mutation was observed in Leptin receptor gene of the Mazandaran native chickens and it is monomorph.