Study on genetic diversity of Xanthomonas oryzae pv. oryzae isolates in rice fields of Gilan province using RAPD marker

Bacterial Leaf Blight of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), is an important disease throughout Asia. Yield losses of 10-70% have been recorded in severely infected fields. In this study genetic variation in 60 isolates of Xoo, investigated using random amplified polymorphic DNA (RAPD) marker. As a whole all used markers amplified 187 polymorphic bands. Analysis of molecular variance of RAPD data showed that the genetic variation within Xoo populations was greater than between populations. The similarity matrix made by Simple Matching coefficient and cluster analysis conducted by unweighted pair group method (UPGMA) with NTSYS-pc soft ware. At a similary index of 0.6 the isolates were grouped into 3 clusters. These results revealed that the RAPD markers which used in this study could differentiate nursery and field isolates from each other.