Acinetobacter and Methalo-β-Lactamase Genes

β- lactamase class B, including IMP, VIM, SPM, the MBLs are read, as of mechanisms to carbapenem resistance in Acinetobacter strains are considered. This caused many problems in the treatment of bacterial infections has created. In this study, evaluate to resistance of Acinetobacter strains isolated from clinical specimens to imipenem and investigation of MBLs genes of blaSPM-I, blaVIM-I, blaIMP- I in these bacteria were done. A total of one hundred strains of Acinetobacter Tehran's Shariati and Baghiyatallah hospital, were collected. The antibiotic suspectibility by diffusion method (disk diffusion) and the minimum inhibitory concentration (MIC) imipenem with microbroth dilution method was conducted. The PCR test strains using gene-specific primers blaSPM-I, blaVIM-I, blaIMP- I was. In total, fifty imipenem-resistant Acinetobacter strains were isolated. The minimum inhibitory concentration (MIC) showed that majority of these strains MIC ≥64μg/ml to imipenem. Investigation of methalo- β- lactamase genes showed that 14% and 26% of species were harbourd blaSPM-I, blaVIM-Irespectively. blaIMP-I gene in these strains were not found According to the results, Acinetobacter strains resistant to carbapenem as a serious problem in patients with these microorganisms is considered. Because of MBLs producing strains of the bacteria, The identification of strains important step in the treatment and control of nosocomial infections caused by these strains is considered.