Evaluation of four Antibiotics Effects on Growth and Their Efficiency to Eliminate Unicellular Alga Dunaliella Sp. Culture Contamination

In present study, Ampicillin, Kanamycin, Vancomycin and Rifampicin concentrations (0.05, 0.1 and 0.3 mg.l-1) efficiency in bacterial contamination elimination of unicellular algae Dunaliella sp. cultures was evaluated. Each antibiotic was able only to reduce contaminant bacterial population in cultures. Combination of two antibiotics (Ampicillin, Kanamycin and Vancomycin) in 0.05 mg.l-1 and 0.1 mg.l-1 concentrations resulted in elimination of bacterial contamination by Ampicillin-Kanamycin mixture in 0.1 and 0.05 mg.l-1, respectively. Algae population changes study by OD680 determination and cell counting showed, except Rifampicin, other antibiotics neither reduced algal growth, also Dunaliella sp. population was higher in presence of Ampicillin compared with control.