Cation Exchange Resin Based on Polybutyl Methacrylate in Determination of Hemoglubin A1c

Determination of glycated proteins is effective in the monitoring of long-term glucose control in individuals with diabetes mellitus. Nowadays micro-column ion-exchange chromatography that could separate the proteins is frequently used. In this system, the separation was carried out on an ion-exchange resin. One of these resins based on polybutyl methacrylate is synthesized, using suspension polymerization technique. The synthesized resin was characterized by analytical methods. FT-IR spectroscopy confirmed presence of carboxylic groups in the resin. Using Scanning Electron Microscope (SEM) and BET surface analysis techniques, respectively, macroporous structure of the resin was confirmed and specific surface area was determined. Application of the resin in separation of hemoglobin A1c was examined by UV-spectrophotometer as detector. It is shown that the synthesized macropore cation exchange resin with cation exchange capacity of 3.083 mEq / (g dry resin) is applicable for Hb A1c hemoglobin separations.