A comparison of the effects of Carbohydrate and Carbohydrate-Protein supplements on heat shock protein 72(HSP72) during intermittent soccer activities

The main purpose of this study was to compare the effects of Carbohydrate and Carbohydrate-Protein supplements on heat shock protein 72 (HSP72) during intermittent soccer activities. 24 soccer players of super clubs were selected and divided into 3 groups of carbohydrate (CHO)، carbohydrate-protein (CHO-PRO) and placebo (P). Intermittent activities included 24 trials of modified Ekblom tests or soccer simulation activities. Subjects ingested drinks before، during and 1 hour after intermittent activities. Blood samples were collected before (baseline)، immediately، 1 hour and 24 hours after simulated intermittent activities. HSP72 levels، Insulin and blood Glucose counts، were measured using Elisa Kit. The results showed significant differences in HSP72 levels، Insulin and blood Glucose levels، immediately and 1 hour after intermittent activities of CHO and CHO-PRO groups compared to placebo group (p ≤ 0 /05). But، there was no significant difference between CHO and CHO-PRO group. In addition، significant increases in HSP72، Insulin and blood Glucose were shown immediately and 1 hour after intermittent activities in CHO-PRO، CHO and P compared to baseline. The results indicated that ingestion of carbohydrate with additional protein before، during and 1 hour after soccer simulation intermittent activities did not attenuate HSP72، Insulin and blood Glucose compared to when the carbohydrate was ingested alone. While، in both CHO-PRO and CHO groups significant differences were shown in HSP72، Insulin and blood Glucose levels compared to P group. Thus، glucose availability during exercise affects the circulating HSP72 response in soccer players.