Comparison of genetic diversity of cytochrome b gene of Shemaya (Alburnus chalcoides) in Haraz, Shirud and Gazafrud rivers by PCRRFLP method

In this study 71 specimens were collected from the Haraz, Shirud and Gazafrud rivers in Mazandaran and Guilan Provinces. The mtDNA was extracted from fish fin using Phenol-Chloroform method. Genetic variation and probable population differentiation of Alburnus chalcoides were studied based on the mitochondrial cytochrome b gene. The specific primers were designed for A. chalcoides and the PCR experiments were performed. Nine restriction endonuclease enzymes were applied for RFLP analysis (BamHI, EcoRV, HaeIII, HincII, HinfI, PpuMI, RsaI, Tsp45I, and TaqI). PCR products (831 bp) and DNA digests were subjected to Agarose gel electrophoresis to separate fragments according to their molecular weight. A part from TagI the rest patterns were identified similar to all specimens from Gazafrud River. Regarding to this patterns, it can be concluded that polymorphism phenomena cannot be observed by above mentioned enzymes and cytochrome b gene, and there is no separate population of A. chalcoides in this study.