Surveillance of current antibiotic resistance among clinical isolates S. aureus, E. coli and P. aeroginosa collected from five Iranian cities

Antibiotic resistance is an emerging problem worldwide present in many bacteria, specially S. aureus, P. aeroginosa, and E. coli that are consider as the most common group of bacteria responsible for nosocomial infections. This problem would be more serious and divesting in developing countries where there is not regular surveillance program for periodic antibiotic resistance. In attempts to report the antibiotic pattern in Iranian cities, we collected clinical isolates and tested them against current antibiotics. 653 Clinical isolates of S. aureus, P. aeroginosa, and. coli were collected during 5 months. The isolates were then transported to research laboratory ithin glycerol containing medium. Standard antibiotic discs containing different concentrations of each agent were provided from MAST media. Standard disc diffusion method was carried out and the result was interpreted using NCCLS tables and data charts. Isolates were mostly cultured rom respiratory, Urine, wounds, blood clinical samples. The clinical isolates were E. coli (45%) S. aureus (33%), and P. aeroginosa (22%). 20% of E. coli, 9% of P. aeroginosa, and 12% of S. aureus isolates showed reduction of sensitivity to Amikacin (AK). 27% of S. aureus had reduction of sensitivity to Vancomycin (VA). About 25% of P. aeroginosa showed reduced sensitivity to Gentamicin (GM).