qacE /qacEΔ1 in Pseudomonas aeruginosa

Pseudomonas aeruginosa is one of the most important infection agents in burn patients. The vast and irregular taking disinfectants and antiseptics such as the quaternary ammonium compound in clinical centers not only did not destroy pathogenic bacteria, but also caused resistance to those compounds in the mentioned microorganism. The goal of this research was to study the susceptibility and presence of resistance genes such as qacE and qacEΔ1 among Pseudomonas aeruginosa witch were isolated from burn patients.

Eighty five Pseudomonas aeruginosa were isolated from clinical sources. Susceptibility of the isolates to biocide containing didecyl diethyl ammonium chloride (Deconex) was determined by broth micro dilution and broth macro dilution. Polymerase chain reaction (PCR) was done for detection of qacE and qacEΔ1 genes.

Among the Eighty five isolates, 37% of isolates showed less susceptibility and 63% of isolates was susceptible to biocide. qacEΔ1 genes were detected in 49.9% of resistant isolates but qacE genes were not seen in any of isolates.

More usage of biocides substances induces resistance genes and cause more resistance to biocides in Pseudomonas aeruginosa. Thus high antibiotic resistance in Pseudomonas aeruginosa and also increase the resistance to biocides substance in these bacteria cause serious problem in cure of Pseudomonas aeruginosa infections.