Detection of Ureaplasma Urealyticum and Mycoplasma hominis in Clinical Isolates from Women with Genital Tract Infection by PCR

The Ureaplasma urealyticum and Mycoplasma hominis are known as sexually transmitted agents, causing mainly urethritis, pyelonephritis, infertility, pelvic inflammatory disease, spontaneous abortion, low birth weight, neonatal meningititis, neonatal pneumonia, infection of the genitourinary tract. The main objective of this study was determined the prevalence of U. urealyticum and M. hominis in women with genital tract infections were collected from Emam Khomeiny hospital at Tehran. In this descriptive study between April 2010 to December 2011, Endocervical swabs from 191 women with genital tract infections were collected from Emam Khomeiny hospital at Tehran. Obstetrician was completed the medical records in reception process. After DNA extraction from isolates, PCR amplification was used for the detection of U. urealyticum and M. hominis respectively. Results were analyzed by SPSS software and statistical tests. From the total number of specimens examined, 58 (30%) clinical isolates were positive for U. urealyticum and 52 (27%) clinical isolates were positive for M. hominis. The isolation rate of bacteria in age group between 31 to 45 years was higher than others. Because of the potential effects of genital Mycoplasma on the success rate of highly specialized treatment, and its causal roles in several maternal complications of pregnancy and in neonatal morbidity and mortality, the rapid detection of U. urealyticum and M. hominis could be important and necessary. More attention needs to be paid to their role as an important etiologic factor of urogenital infections.