Evaluation of Cellular Toxicity of Three Denture Base Acrylic Resins

This study aimed to evaluate the cellular toxicity of two newly-released acrylic resins (Futura Gen and GC Reline Hard) in comparison with the conventional heat-cure resin (Meliodent). Sample discs from each acrylic resin were placed in 24-well culture plates along with L929 mouse fibroblast cell line. A mixture of the RPMI 1640 medium, antibiotics and 10% FBS was added to the plates and the specimens were incubated in a CO2 incubator. The amount of light absorption by each plate was determined after 1 hour, 24 hours and 1 week by the MTT assay and ELISA. The cytotoxic effect of the resins was compared among groups using the two-way ANOVA and further paired comparison was performed using the post-hoc Tukey’s test. After 1 hour, Futura Gen displayed a significantly lower level of light absorption in comparison with Meliodent (P=0.03). After 24 hours, GC Reline Hard rendered a significantly lower level of light absorption compared to Melio-dent (P=0.02). After one-week of incubation, the mean absorption rates for GC Reline Hard, Meliodent and Futura Gen were relatively similar (P>0.05). The lowest and highest level of cytotoxicity among all resins were observed after one week and 1 hour of immersion in water, respectively. All the tested resins induced some degree of cytotoxicity. Cytotoxic-ity of Futura Gen, GC Reline Hard and Meliodent resins failed to show any sig-nificant reduction from 24 hours to one week. Thus, it is recommended to im-merse the dentures in water for 24 hours prior to delivery to the patient.