Functional analysis of AT1G77640 in Arabidopsis

ranscription factors are important regulators of gene expression in all biological processes and are important in genetics too. Based on our results using qRT-PCR technique, AT1g77640 gene belongs to the AP2 transcription factor family, involves in cell cycle control in Arabidopsis. To identify biological processes regulated by AT1g77640 in detail, molecular characterization of transgenic plants with modified levels of AT1g77640 expression (constitutive over expression and amiRNA) was performed using profiling technology. Additionally expression pattern of the studied TF was analyzed using promoter-GUS lines and publicly available microarray data. Consistent with our data activation of AT1g77640 in cell cultures had fluctuation. Activity of AT1g77640 gene in the cell suspension decreased in late G2 and M phases. Constitutive over expression of AT1g77640 in Arabidopsis influenced cell size and cell number and also decreased seed production. The expression pattern of the gene was determined by qRT-PCR and through GUS fusions, indicated preferential transcriptional activity including flower buds, hypocotyls, vascular tissues, cotyledon and auxiliary meristem.