Frequency of Mycoplasma hominis and Ureaplasma urealyticum in Females With Urogenital Infections and Habitual Abortion History in Ahvaz, Iran; Using Multiplex PCR

Infections due to Mycoplasmatales members can cause infertility, preterm delivery and neonatal morbidity and mortality. Therefore, rapid diagnosis is of great importance to control these infections and diminish their outcomes.. The current study aimed to develop the multiplex PCR assay to detect two genital mollicutes from a single amplification reaction and the study of their relation with habitual abortion and urogenital infection in infected females.. Urine and genital samples from symptomatic females (20-54 years old) in Imam Khomeini hospital, Ahvaz, Iran, were screened for the two common mollicutes, Mycoplasma hominis and Ureaplasma urealyticum; using multiplex PCR. The prevalence of these mollicutes in 200 healthy females was also evaluated.. The results of the current study showed that the presence of M. hominis in urine (P = 0.006) and genital samples (P = 0.01) was associated with urogenital infections. Statistical analysis based on the age, revealed that the highest prevalence of M. hominis (71.4%) and U. urealyticum (60%) were in females within 30-34 and 35-39 years old, respectively. Similarly, in genital samples, the highest incidence of M. hominis (54.5%) and U. urealyticum (53.8%) was found in females within 28-33 and 34-39 years old. Interestingly, further analyses revealed direct strong relation between the mollicutes used in this study and habitual abortion as well as urogenital infections.. There was a strong relation between the presence of the studied M. hominis and U. urealyticum with urogenital infection in the females under study in comparison with those of control groups. The studied mollicutes were highly associated with habitual abortion in symptomatic females. The multiplex PCR was developed for simultaneous, early, and easy detection of these potential pathogens..