Antioxidant and antimicrobial activities of the methanolic extracts of three Trigonella species from Iran

This paper represents a comparative study of total phenol and flavonoid contents، antioxidant and antimicrobial activities of the methanolic extracts of three Trigonella species، namely T. disperma، T. teheranica and T. subnervis. Total phenol and flavonoid contents were determined by Folin Ciocalteu and Aluminum chloride colorimertric methods، respectively. DPPH free radical scavenging assay was used to evaluate antioxidant activity. In addition، antibacterial activities of the extracts against six gram positive and negative bacteria were studied by disc diffusion method. Total phenol and flavonoid contents varied from respectively 8 ± 0. 28 to 22. 2 ± 1. 4 mg/g dw and 5. 23 ± 0. 33 to 10. 48 ± 1. 2 mg/g dw، in the studied species. In addition، the species showed high antioxidant capacities. However، there were no significant differences between their IC50 values. The highest scavenging potency of DPPH free radical was observed in T. subenervis with IC50 = 0. 123 ± 0. 006 mg/ml. All three species، especially T. teheranica showed remarkable antimicrobial activity against some studied bacteria.