Detection of vitellogenin gene expression changes in liver of juvenile Persian sturgeon (Acipenser persicus) exposed with nonylphenol Xenoestrogen compound

The present study demonstrated the effects of nonylphenol on Persian sturgeon (Acipenser persicus) vitellogenin (VTG) gene expression changes in juvenile Persian sturgeon liver with RT-cPCR and RT-qPCR techniques. For study of vitellogenin gene expression and 18S rRNA as a control gene expression, the liver of juvenile Persian sturgeon injected three times with 17bestradiol (5 mg/kg body wt/week) or NP (1, 10 and 100 mg/kg/week) in three weeks; have been used. Significant induction of VTG gene expression was detected in all treated groups in comparison with control group. According to this research, the ratio of VTG to 18s rRNA in group receiving 1 ßestradiol was 9.95±2.48 for VTG. All groups treated with nonylphenol have significant effect on expression of VTG gene by analyzing statistical software. These results are the first report for effect of the risk of xenoestrogen on Persian sturgeon and vitellogenin gene efficiency as biomarkers of xenoestrogens in Persian sturgeon have been accepted.