Detection of precocity gene using RAPD marker in tobacco (Nicotiana tabacum L.)

One of the limits for tobacco (Nicotiana tabacum L.) farming extension is short time for growth in many parts of the world. One solution to this issue is the use of precocious cultivars. The plan was performed to determine suitable molecular markers for detection of precocity gene on the basis Bulk Segregation Analysis. To achieve this goal, a precocious cultivar (Urumieh 2) and a serotinous varietie (Burley Ree 103) from the Burley type was crossed and the F2 generation was grown on the farm. One hundred plants were selected, contained precocious and serotinous. This plants was sampled and DNA was extracted. The annealing temperatures of 60 RAPD primers were determined and were tested on parents and precocious and serotinous Bulks. Two primers (OPC09 and OPB08) showed polymorphism and after the test on 96 samples from the F2 generation two markers were selected (OPB08- 1050 and OPC09-1900). That showed 19.7 cM and 15 cM distance from precocity locus respectively.