Investigation of phylogenetic relationship of Barbinae species in south of Iran

to investigate the phylogenetic relationship of Schizothorax zarudnyi, Schizocypris altidorsalis, Barbus sharpeyi B. subquinciatus, B.xanthopterus and B.esocinus, the native barbinae species South of Iran, 10, 10, 5, 3 and 4 samples were collected from Chahnemeh’s reservoirs and Karoon River and transferred to laboratory. Genomic DNA was extracted from fin tissue by phenolchlorophorm method. PCR was performed using pair specific primers to amplification 980 bp mitochondrial cytochrome b (Cyt b) region. After checking the PCR products by electrophoresis, then they purified using purification kit and directly sequenced. Results showed that Schizothorax zarudnyi and Schizocypris altidorsalis form a monophyletic group that is sister group with Barbus sharpeyi than other species. Based on comparison Cyt b sequences of all barbus species suggested that Iranian barbinae were paraphylitic than other species. In fact, this position reflected that variation and diversion in Iranian barbinae take place in long time and independently than other barbinae in the world. The reported results could be of interest for management and conservation programs of this valuable native species.