Synthesis and In-vitro Cytotoxicity Assessment of N-(5-(Benzylthio)-1,3,4-thiadiazol-2-yl)-2-(4-(trifluoromethyl)phenyl)acetamide with Potential Anticancer Activity

Cancer is the second leading cause of death in the world. Despite advances in the diagnosis and treatment, overall survival of patients still remains poor. Hence, there is an urgent need for development of new anticancer agents. Considering promising biological activity of 1,3,4-thiadiazole derivatives, in the present study, synthesis and cytotoxicity assessment of new derivatives of this ring was done. All synthesized compounds were characterized by NMR, IR and MS spectroscopic methods. Obtained data from MTT assay showed that all compounds 3a-3l hadbetter anticancer activity against MDA(breast cancer) compared to PC3(prostate cancer) and U87(Glioblastoma).Compound 3g with m-OCH3 moiety on the phenyl ring was the most potent one in this series with IC50 = 9 µM against MDA breast cell line in comparison with imatinib(IC50 = 20 µM) as reference drug.