Evaluation of SSR and ISSR markers on 1D locus of bread wheat mutant lines associated with desirable bread-making quality

Aset of 40 F2 bread wheat from two crosses between parents with desirable bread making quality (Tajan) and two mutant lines from Tabasi with high yield and undesirable bread-making quality (T- 66-58-9 and T-66-58-12) screened and used to investigate SSR and ISSR markers related to desirable bread-making quality. Therefore, the aim of this study was accomplishment to microsatellite and designed inter simple sequence repeat markers linked to Glu-1D genomic locus for identification of bread wheat mutant lines. The two parents were analyzed with 2 SSR primers pairs and 6 ISSR primers. Of these, 2 primers (25%) were showed high polymorphism between two parental genotypes each cross. Using these primers, electrophoresis results of F2 progeny were analyzed and mean of similarity coefficient were computed for each progeny. Primers, S2 and S13 showed very high polymorphism percentage and power of discrimination. These markers may be useful in genotyping and genetic diversity studies for the genetic improvement program of bread wheat cultivars and mutant lines.