Bioinformatics analysis of the BMP15 exon 2 in Tali and Beetal goats

Using molecular technology has resulted in discovering the mutations with major effect on reproduction efficiency in goat. Because of the importance of litter size in goats, in this research, the mutations found in previous research and new mutations were investigated in Beetal and Tali breeds for BMP15 gene. After DNA extraction from blood samples of Beetal and Tali goats and PCR reactions, nine PCR products (from individuals with different kidding records) from mention breeds were sequenced. In Beetal goats no nucleotide change has been found for BMP15 gene. Also, a heterozygote mutation was found at position 807 in Tali that led to replacing a glutamine amino acid with a glutamic acid amino acid (Residue of 269). Due to the replacement of an uncharged amino acid with a charged amino acid, it is theorized that this mutation disrupts dimerization and leads to change in the electrostatic surface potentials of this loci and hence abolish biological activity.