Serological and molecular detection of two mosaic borne viruses in maize fields of Golestan province

In the recent years, symptoms of viral diseases such as mosaic, stunt and yield reduction has been observed at corn fields. Maize dwarf mosaic virus (MDMV), in the north, and Iranian Johnsongarss mosaic virus (IJMV), in the most regions of Iran, are important viruses in corn fields. For detection of these viruses from Golestan province corn fields, 350 plant samples from the fields were selected and then samples survived from DNA-ELISA with specific antiserums of two viruses and RNA production is accomplished. Extracted cDNA were replicated by specific primers at PCR test. Results of ELISA test showed that corn fields are infected to two viruses simultaneously in the some regions. That some of the corn samples response positive. Also infection of MDMV observed in samples of zea and Johnsongrass. All of studied regions are infected to IJMV. Positive responses in PCR test with used primers supported the results of ELISA test and existence two section of reproduction fore per virus in electrophoresis showed simultaneously infection samples for two viruses. Also results of PCR product electrophoresis from symptoms infected to Millet-pearl show only section of MDMV. For result of this study the most zea, johnsongrass with mosaic, yellow and stunt infected to one or both of IJMV and MDMV.