Investigating the genetic variation in the collection germplasm Sistan grapevine native and non-native cultivars using REMAP marker

The aim of this study Investigating the genetic variation in the collection germplasm Sistan grapevine native and non-native cultivars using REMAP marker. To study the genetic diversity of grapevine native and non native cultivars of Zahak research station in Sistan, 33 grape varieties (Vitis vinifera L.) were evaluated by by 7 primer combination REMAP combination ISSR primers and retrotransposons based primer from of Ty1-copia and Ty3-gypsy families. The observed polymorphism for markers MS1 / Gret1 Ra, MS5 / Gret1Rb in both native and non-native varieties were 80 and 75%, respectively. The results revealed that the Gret1 retrotransposon has more transcription and translocation trough the genome. Cluster analysis based on Jaccard's similarity and UPGMA algorithm showed that the population is divided into two groups at (0.44) similarity; one group with twelve sub-groups and one group with one varieties. The results of principal component analysis extracted six components that explained 50.44 % of the total variation among the studied population.