Study of Genetic Relationships of Some Mint Species Using ISSR Markers

Mentha species is a genus of family Lamiaecae، and is well known for its great medicinal and economic values. in order to evaluate the genetic variation of 15 genotypes belonging to three species M. longifolia، M. pulegium and M. Sp.، used were from 10 ISSR primer. The average percent of polymorphism in all accessions was 94. 70. The primers of IS11 & IS9 having the best primers parameters were introduced in this study. Low coefficient of similarity based on Jaccard (0. 17 - 0. 56) have showed a high genetic diversity of Studied genotypes. Cluster analysis based on Jaccard coefficient and UPGMA method classified the genotypes in tree major groups and the PCoA analysis confirmed the results of clustering. Analysis of molecular variance (AMOVA) among and within species showed more intra-specific variation (98. 00%) in comparison with inter-species variation (2. 00%). Evaluation of genetic diversity within species with an average of Nei’s gen diversity analysis and Shannon’s information index، showed that diversity within species of M. longifolia (H=0. 165، I =0. 460) was o more than other populations while genetic diversity within species of M. sp. (H=0. 102، I=0. 194) was less than other species. The results showed that the overall addition to geographic distance، gene flow between populations in the wild populations، ecological conditions is determinating genetic distance and genetic diversity. Also because hybridization between species during evolution of mint،is similarity between species than within species similarities.