The survey of IRFI005 function about removing of intra and extracellular free radicals

Antioxidants are molecules that inhibit the action of free radicals and vitamin E is one of the most important antioxidant compounds. Vitamin E is insoluble in water and it will limit its application in polar media. Many studies are performing for finding of water-soluble analogues of this compound at present. In this study, polar and synthetic analogue of vitamin E (IRFI005) was used for survey the expected properties of vitamin E in polar media. Evaluation of IRFI005 extra-cellular antioxidant activity was studied by spectrophotometric method at 430 nm and by using the Galvinoxyl free radical in 1:2 and 1:4 (IRFI005: Galvinoxyl) molar concentrations. Intra-cellular antioxidant effects of 5 µM IRFI005 was also studied by using DCFH2-DA colored probe and cumene hydro peroxide in DCF method. IRIF005 inhibits the oxidant molecules in the inside and outside media of the cell. Galvinoxyl has lower optical density (OD) in ratio 1:2 and it has been removed completely from the media. Each IRFI005 molecule is able to remove two free radical molecules. It also suppresses CHP effect into the cell and inhibits the increase of fluorescence intensity. The findings suggest that in polar media, IRFI005 is a powerful, dose-dependent and active antioxidant and it can inhibit oxidant molecules of inside and outside the cell.