meticillin resistenance in coagulase negative staphylococci isolated from laryngoscope in Shahid Rajaei hospital, Qazvin Iran

Oxacillin is commonly used in the treatment of beta-lactamase resistant staphylococci which causing several clinical infections. In vitro routine test for Oxacillin resistance may fail to detect due to presence of the mec(A) gene resulting in treatment failure. twenty seven staphylococci isolates were subjected to routine antibiotic susceptibility testing including Oxacilin (1µg) by kirby- bauer disk diffusion method. The Oxacillin resistance was detected by screen agar dilution (4% NaCl + ox 6 µg / ml in M.H.A/24 h /33-350c) and then with PCR for mec (A) gene. In total, 5 isolates (17/86%) were resistant to Oxacillin. Finally, two coagulase negative staphylococci isolates(7/14%) carried mec (A) genes. This study showed that handle of laryngoscope can be contaminated with resistant isolates. Considering the notable Oxacillin resistant these isolates cleaning and use of appropriate disinfectant is necessary to decrease the spread of them by laryngoscope in the special wards of hospital. Keywords: Laryngoscope, staphylococci, D-test