Determination of Yield Components of Trigonelline in Fenugreek (Trigonella foenum-graceum L.) Seed Based on Path Analysis and Regression

Trigonelline in fenugreek seed is a valuable medicinal metabolite in reducing blood cholesterol and blood. Object: Determine the most important components of seed yield, percent and yield of seed trigonelline in fenugreek under foliar application and non-application of methanol. The experiment was done a randomized complete block design with two treatments as foliar application of methanol (0 and 30 v/v%) and four replications. Path analysis and stepwise regression were used in order to denote the most important effective traits on seed yield, percent and yield of seed trigonelline in fenugreek. The results showed that 1000-seeds weight and number of branches per plant for seed yield; total dry weight and number of pods per plant for percent of seed trigonelline; seed yield and percent of seed trigonelline for yield of seed trigonelline in the non-application of methanol, and also, the number of seeds per pod, percent of seed oil, number of pods per plant and plant height for seed yield; leaf dry weight, shoot trigonelline and geometric mean diameter of seed for percent of seed trigonelline and seed yield; percent of seed trigonelline and pod length for yield of seed trigonelline in foliar application of methanol (30 v/v%) treatment significantly justified changes related to the dependent variables. Path analysis of dependent variables showed that the yield of seed trigonelline by direct positive effect of seed yield and the percent of seed trigonelline were the most important yield components of seed trigonelline. Generally, the results indicate that seed yield and percent of seed trigonelline can be used for increasing of yield of seed trigonelline in the breeding of fenugreek.