Development of Vacancy Solution Theory Based on Local Composition Concept for Protein Extraction Using Reverse Micellar System

Three thermodynamic models were developed by combining vacancy solution theory and local composition concept with the aim of phase behavior study of biomolecules in separation process using reverse micellar system. Also, to check the capability of the developed models, each one has been applied to the experimental data of reverse micellar extraction of bovine serum albumin were composed of cetyl tri methyl ammounium bromide surfactant. The results showed that the developed models are able to express the non-ideality of the system due to the variation of aqueous pH. Moreover, these models are able to follow the increasing trend of protein extraction due to increasing of surfactant concentration. Furthermore, the results showed that the calculated protein partition coefficients are in good agreement with the experimental data.