The Biosynthesis of Silver Nanoparticles using OAK fruit extract and the investigation of their Anti-microbial Activities against nosocomial infection agents

Bacteria is the most of nosocomial infection creating agent that has created many problems for human health by resistant to conventional antibiotics. Silver nanoparticles have high antimicrobial properties which can be used in various fields of medicine and public health control. The purpose of this study was to produce silver nanoparticles with single-stage Biological method through applications of oak fruit extract and investigate its anti-bacterial activity. the extract of oak fruit was prepared and added to the silver nitrate solution. The biosynthesis of silver nanoparticles in solution was investigated through checking color change and the degree of absorption in different wavelengths by spectrophotometer. The Anti-microbial Activities of the Silver nanoparticles in solution against Escherichia coli PTCC 1330، Staphylococcus aureus PTCC 1112، Pseudomonas aeruginosa PTCC 1074 و Bacillus subtilis PTCC 1715 bacteria was investigated by inhibition zone method. The color changing was observed from transparent to deep brown in the solutions. the degree of absorption was increased and in 420 nm maximum absorption was observed. The antibacterial activity against all bacteria was investigated and approved. Discussion & the Silver nanoparticles was synthesised in a single-stage and little time and showed suitable antibacterial activity.