Comparison PCR and Culture methods for detect Coccoid forms of Helicobacter pylori
Author(s):
Abstract:
Background
Helicobacter pylori (Pylori) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. Environmental factors such as water a reservoir of H.pylori which infect human. a Non-culture bacteria in coccoid forms widespread in aquatic environments.The objective of this study was elevating the diagnostic value of PCR and culture methods for for diagnosis coccoid forms of H.pylori.Materials And Methods
To induce coccoid forms, ten different strains of H.pylori (H1-H10) were inoculated into 30 drinking water samples. Then, the samples were incubated at three different temperatures of 4°C, 22°C and 37°C for the durations of 30 and 60 days. The samples were cultured on brucella blood agar and DNA was also extracted also from them and PCR performed on samplesResults
percentage of H.pylori cells detected at specified temperatures by the culture were 0%, 10% and 0% in the first month and were 0%,10%,30% in the second month whereas by the PCR molecular method were 30%, 80%, and 30% in the first month and were 20%, 20%, and 40% in the second month, respectively.Conclusion
finding show PCR methodis more capable than culture fordetect the coccoid forms of H.pylori, therefore this method could be used to detect non- culture forms.Keywords:
Helicobacter pylori , coccoid , PCR , Culture
Language:
English
Published:
Govaresh, Volume:19 Issue: 3, 2014
Pages:
175 to 181
magiran.com/p1321238
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!