Curcumin-loaded Chitosan Tripolyphosphate Nanoparticles as a safe, natural and effective antibiotic inhibits the infection of Staphylococcus aureus and Pseudomonas aeruginosa in vivo

Curcumin as an antibacterial agent is a yellow natural compound extracted from turmeric root. Nanoparticles can decrease the defects of usual drug delivery systems. Chitosan is a low toxic, biodegradable, biocompatible and safe polymer which is used in production of nanoparticles. Nanoparticles including chitosan-tripolyphosphate (TPP) can increase antibacterial properties of curcumin. Curcumin loaded chitosan-TPP nanoparticles were synthesized by ionotropic gelation method from chitosan, curcumin and TPP salt. The skin of anesthetized mice was inoculated with staphylococcus aureus and pseudomonas aeruginosa. The infected mice were treated with curcumin loaded chitosan-TPP nanoparticles during 3 days. Antibacterial characteristics of curcumin-nanoparticles treated mice were evaluated by bacterial culture of infected mice. Our results showed the size of 160 ± 10 nm and the charge of +7 ± 2 mV in curcumin loaded chitosan-TPP nanoparticles. These nanoparticles were also spiral shape. The encapsulation efficiency of curcumin loaded chitosan-TPP nanoparticles was 75 ± 2%. Bacterial culture showed that curcumin loaded chitosan-TPP nanoparticles inhibited staphylococcus aureus and pseudomonas aeruginosa growth. Our study demonstrated that curcumin loaded chitosan-TPP nanoparticles can be utilized as a potent agent in treatment of Staphylococcus aureus and Pseudomonas aeruginosa infections.