Study of Root Inoculation with Plant Growth Promoting Bacteria (PGPB) and Spraying with Their Metabolites on Chlorophyll Content, Nutrients Uptake and Yield in Rice (Hashemi cultivar)

In order to study the spraying of Plant Growth Promoting Bacteria (PGPB) and their metabolites on chlorophyll content، nutrients uptake and yield in Hashemi cultivar of rice [u1]، an experiment was arranged based on completely randomized block design with four replications in greenhouse of at the University of Guilan during 2010. In this research، the examined treatments consisted: without spraying and fertilizer (control)، without spraying and use of fertilizer، sprayingwith P. fluorescens strain 168، sprayingwithmetabolites of P. fluorescens strain 168، root inoculation with P. fluorescens strain 168، sprayingwith P. fluorescens strain 136، sprayingwithmetabolites of P. fluorescens strain 136، root inoculation with P. fluorescens strain 136، sprayingwith P. fluorescens strain 41، sprayingwithmetabolites of P. fluorescens strain 41، root inoculation with P. fluorescens strain 41. The results of experiment showed the effect of bacteria different levels was significant on leaf greenness content، calcium، magnesium، iron، yield and harvest index، but wasn’t significant on chlorophyll a، b and carotenoid contents. [u2] Root inoculation with P. fluorescens strain 41 was superior to the other treatments regarding all of the studied indices. However، the spraying with P. fluorescens strain 41 had lower effect than the root inoculation with P. fluorescens strain 41 and P. fluorescens strain 136، but its results was better as compared to the fertilizer and non-sprayed treatments. Spraying effect of metabolites from various bacteria was different that may explain the various bacterial regulator effect on plant growth and development. Results showed that root inoculation with growth promoting bacteria was successful as compared to their spraying in terms of quantitative and qualitative indices of rice. Spraying، however، can be used as complementary to root inoculation with PGPR for crop yield improvement.