The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis
Author(s):
Longyu Zhang , Ying Liu , Yuping Xu , Huan Wu , Zhaolian Wei , Yunxia Cao
Abstract:
Background
To investigate the expression of Beclin-1 mRNA and protein in eutopic and ectopic endometrium of women with and without endometriosis, and evaluate the association of Beclin-1 protein expression and serum CA125 levels in the endometriosis group due to CA125 being a well-known biomarker of endometriosis. Materials And Methods
The expression levels (mean ± SD) of the mRNA and protein of Beclin-1 were examined in uterine endometria from 26 women without endometriosis and in eutopic and ectopic endometria from 26 endometriosis patients through experimental study, as reverse transcription PCR and Western-blotting assays. Serum CA125 levels in the endometriosis and control groups were compared and the correlation between Beclin-1 protein expression and serum CA125 was evaluated in the endometriosis group. Results
Both eutopic (0.12 ± 0.04, 1.25 ± 0.42) and ectopic (0.12 ± 0.05, 1.09 ± 0.50) endometriotic tissue from 26 women with endometriosis expressed significantly lower levels of Beclin-1 mRNA and protein than endometrium from 26 normal women (0.15 ± 0.02, 1.67 ± 0.44) (p<0.05). Serum CA125 levels were found to be significantly higher in the endometriosis group (p<0.05). In addition, Beclin-1 protein expression of eutopic endometria in patients with endometriosis was negatively correlated with serum CA125 (r= -0.57, p<0.01). Conclusion
The present study strongly suggests that Beclin-1 may play a role in the formation and progression of endometriosis.Keywords:
Autophagy , Endometriosis , Beclin , 1 , CA125
Language:
English
Published:
International Journal Of Fertility and Sterility, Volume:8 Issue: 4, Jan-Mar 2015
Page:
429
magiran.com/p1362172
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!