Cardiopulmonary Exercise Testing (CPET) in Moist Environment, and Its Relation to the Selected Markers of Salivary Oxidative

Asthma and obesity are asso-ciated with breathing problems. Exercising increases the probability of the production of harmful free radicals by increasing oxidative stress; on the other hand, it decreases free radicals by stimulating anti oxidative enzymes. There is insufficient information about the evaluation of cardio – respiratory function…. in asthmatic chi-ldren doing acute exercise in humid env-ironment, and also about its relation to Salivary oxidative and anti-oxidative fact-ors. The purpose of this study was to inves-tigate the effect of aerobic and exhaustive exercises on cardio-respiratory indices, and its relation with selected markers of salivary oxidative stress in asthmatic obese children in comparison with healthy children. 10 asthmatic obese children (BMI 25.12 and fat 29.86%), and10 healthy obese children (BMI 26.8 and fat 31.49%) did a progressive and exhaustive exercise in a constant tempera-ture environment 2 ± 22 °C, and humidity 65 ± 5%. Saliva samples were collected to determine the levels of Maloen dialdehyde (MDA) and superoxid dismutase(SOD) in two stages, before and after exercise. During exercise, the steady-state levels of cardio-respiratory parameters, such as VO2 peak, oxygen pulse (OP), ventilation thres-hold (VT), were controlled using K4B2. Heart rate (HR) was measured by the rate Polar gauge during exercising. Doing exhaustive exercise caused an insignificant decrease in MDA in asth-matic obese children (P = 0.366), and in healthy obese children (P = 0.127). It also caused an insignificant increase in SOD saliva in asthmatic obese children (P = 0.268), and in healthy obese children (P = 0.092). Ventilation threshold in asthmatic obese children was significantly lower than healthy obese children (P <0.005). There wasn´t any significant difference in cardio-respiratory indices)HR,OP,VO2peak (betw-een asthmatic obese children and healthy obese children. An insignificant negative correlation between MDA levels and cardi-ovascular parameters (HR, VT, OP) and SOD with OP was observed, and there was an insignificant positive correlation betwe-en SOD and HR, VT. Discussion & These results indicate that, although, the mild to moderate asthma is associated with salivary oxidative stress and cardiorespiratory dysfunction in the children of present study, but it just dec-reases the ventilation threshold consi-derably. However, further studies should be performed in order to identify the effect of cardio-respiratory exercise intervention on salivary oxidative stress in asthmatic children.