Determination of Antibacterial Activity of Pistacia lentiscus Methanolic Extract on Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguis, Pseudomonas aeruginosa

There are different species of bacteria in different parts of oral c-avity that cause the tooth decay and pe-riodontal diseases. Pistacia lentiscus L. has been used for gingival problems, to-othache and aromatizing the mouth in Iranian traditional medicine. The aim of this study was evaluating the antibac-terial effect of Pistacia lentiscus L. on Staphylococcus aureus, Streptococcus mutans, Streptococcus sanguis and Ps-eudomonas aeruginosa. Methanolic ex-tract of Pistacia lentiscus L. from 100 to1000 mg/ml dilution were examined on strains of Staphylococcus aureus (ATCC25923), Streptococcus mutans(ATCC1601), Streptococcus sanguinis (ATCC1449) and Pseudomonas aerugi-nosa (ATCC25923). The well assay an-d disc diffusion methods were applied for testing the antibacterial activity of Pistacia lentiscus L. The Minimum Inh-ibitory Concentration (MIC) and Min-imum Bactericidal Concentration (MB-C) were detected. The Streptococcus mutans an-d Pseudomonas aeruginosa were not se-nsitive to Pistacia lentiscus L. Staphyl-ococcus aureus and Streptococcus sang-uinis were sensitive at 50, 100 and 1000 mg/ml concentrations. With disk diff-usion method, sensation to Pistacia lent-iscus L. was only showed at 100 mg/ml concentration for Streptococcus sangu-inis that produced an inhibition zone with 17 mm diameter. The MIC of Pist-acia lentiscus L. for Streptococcus sang-uinis was 1000 mg/ml. The other bacte-ria grew in all concentrations. Discussion & The meth-anolic extract of Pistacia lentiscus L. in some concentrations has antibacterial effect on Staphylococcus aureus and St-reptococcus sanguinis, but it doesn´t have any antibacterial effect on strept-ococcus mutans and Pseudomonas aeru-ginosa.