Detection and distribution of the important viruses infecting Damask rose in floricultures of Isfahan, Markazi and Kerman provinces

Prunus necrotic ringspot virus (PNRSV)، Apple mosaic virus (ApMV) and Arabis mosaic virus (ArMV) are important rose viruses in the world. The aims of this study were to detect the viruses and evaluate their distribution in damask rose floricultures of the Isfahan، Kerman and Markazi Provinces. To do this، total 749 leaf samples were collected randomly from floricultures of the mentioned provinces in 2012-13. Presence of the viruses in the samples was checked by DAS-ELISA using specific antibodies against each of the viruses. The result of ELISA test on collected samples in 2012 showed that 4. 41%، 2. 20% and 6. 3% of the samples were infected with ArMV، ApMV and PNRSV، respectively. These percentages for samples collected in 2013، were 2. 31%، 5. 32% and 4. 16%. Because of wider distribution of PNRSV in comparison to ArMV and ApMV، coat protein gene in four isolates of PNRSV was cloned and sequenced. Phylogenetic tree was drawn and recombination analyses were performed. Based on the phylogenetic tree، Iranian isolates were placed in PV96 phylogroup. This is the first report of genetic diversity of PNRSV in Iran and also the first incidence report of ArMV، ApMV and PNRSV in Isfahan and Markazi provinces.