Expression of genes related to macromolecule metabolic process under cold stress in almond (Prunus dulcis Mill) using RNA-seq analysis

RNA-seq is revolutionizing our ability to characterize abiotic stresses at the transcriptomic levels. In present study، using RNA-seq analysis، we tried to demonstrate the major processes related to cold stress of almond ovary. Results showed that the number of 358 differential expressed genes were involved in macromolecule metabolic process. Besides، to functional analysis of these genes، we determined the domain type of above mentioned genes. Results suggested that these genes have different combination of domains including pkinase، LRR، AP2، phytocorome and many other domains. Many of the detected domains and protein kinases were previously reported in different cold inducible and cold tolerant proteins. In addition، the highly over expression of 40S ribosomal proteins and NAC، sugessts that at least some partion of these genes are associated with cold stress resistance، and might have great potential to be applied in breeding programs for abiotic stress tolerance in almond and other Rosaceae species.