Expression pattern of GmOSBP, CAT and GmBZIP genes under saline stress in soybean (Glycin max L.)

GmOSBP and GmBZIP genes play a key role in signaling of response to certain physiological repercussion to stress and CAT gene plays a role through inhibition of ROS in plants This research was conducted to evaluate the expression profile of GmOSBP، CAT and GmBZIP genes in two soybean cultivars، Williams (tolerant) and L17 (susceptible) at salinity condition. Two soybean cultivars were cultured based on completely randomized design with three replicate in the greenhouse conditions، and salinity stress including zero (as non stress) and 300 mM NaCl treatments was performed at five-leaf stage plantlets for 7 days. The sampling from both from leaves and roots for two cultivars was performed in the same time at five-leaf stage. Total RNA was extracted from leaves and roots of both control and stressed plants. Then cDNA was synthesized and used for Real time PCR in two replications. To normalize data the housekeeping gene 18SrRNA was used. Data analysis based on Ct observations showed that the expression of three genes was increased under salinity stress in both leaf and root organs. The expression of GmBZIP gene in Williams was two-fold greater than L17 cultivar and its expression was higher in root than leaf. Also GmOSBP gene expression in L17 was two-fold greater than Williams’s cultivar and its expression was tree-fold greater in leaf than root. The expression of CAT gene in L17 was tree-fold greater than Williams and its expression was higher in root than leaf. According to increasing of salinity tolerance through the expression of these genes، it can be concluded that transferring of these genes may enhance salinity tolerance in other crops.