Study on the polymorphism in myostatin gene and its association with growth traits in Kordi sheep of Northern Khorasan

Effective myostatin gene or factor 8 on growth and differentiation operates as an inhibition moderator in growth and development of skeletal muscles. In the resent research، the blood sampling was randomly carried out of 58 Kordi sheep of Northern Khorasan and then the DNA extraction was done by optimized salting-out procedure. A 337 base-pair fragment from exon 3 of Myostatin gene was replicated using a couple of specific primer by polymerase chain reaction (PCR). It was used of restriction enzymes HaeIII، in order to tracing of different allelic forms of the Myostatin position by PCR-RFLP method. Of three possible genotypes in exon 3 of GDF8، it was observed only two genotypes of Mm and mm that their abundance was 0. 15 and 0. 85، respectively. Allelic frequency for M and m alleles was estimated 0. 08 and 0. 92، respectively. The analysis of linear models with fixed effects of genotype shows that the weight of lamb’s average in 3 and 6 months of heterozygote genotype is higher than the homozygote.