Evaluate the Effects of Bee Venom on Toxoplasma gondii tachyzoites: In-Vitro

Epidemiological studies shown that people were exposed to bee venom, much less affected parasitic diseases. This study measured anti-parasitic Effect of bee venom in cell cultures infected with Toxoplasma gondii tachyzoites.Matherials and Human foreskin fibroblasts (HFF) for 10 culture were selected. The T.gondii tachyzoites and bee venom was added to the 8 culture flasks. After 24 h incubation at 37 ° C, the growth of tachyzoites were examined by microscopy. For the control group, a flask without tachyzoites with bee venom 4 μg/μl and flask with cell line was used. Appropriate bee venom concentrations were obtained using the XTT test. Results showed that 4 μg/μl of bee venom has no effect on HFF healthy cells. Bee venom inhibits 70% tachyzoites growth (P <0.01) and had anti-parasite T. gondii tachyzoites effect. significant differences between grows two groups observd (P <0.05). It result was also shown that 64 μg/μl of bee venom, will inhibit the HFF cells growth.Conlusion: According to this study, the anti-parasitic effects of bee venom clearly visible. Bee Venom for the destruction of T. gondii tachyzoites at a suitable concentration was very strong and recommended tested in animal models.