Virulence of Different Strains of Agrobacterium rhizogenes on genetic transformation in Astragalus compactus
Astragalus compactus L. seeds were collected fromTehran of Iran. Plants were grown in growth chamber under 16 h daily light periods and leaves, stems, prdicle and cotyledon leaves explantn from three- week- old sterilized seedlings cut and infected with Agrobacterium rhizogenes Strains 15834, 2659 and WT.Hairy roots obtained from leave and stem explants but did not induce hairy roots of cotyledon and pedicle explants of Astragalus compactus.Strain 2659 resulted in the highest amount of hairy root formation in this Species. Strain 15834 was beneficial but not as effective as strain 2659. In contrast to these two strain, strain WT did not induce hairy roots in any of the explants used in this study. Thus, this strain seems to be totally avirulent to Astragalus compactus.
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