Development of methemoglobin-based biological dosimetry in gamma- irradiated mice
Author(s):
X.H. Zhang , Y.N. Zhang , X.Y. Min , Z.C. Lou , A.L. Wang , X.D. Hu , Dr. H.Q. Zhang
Abstract:
Background
A new biological dosimeter based on methemoglobin level was developed in this study. Materials And Methods
Methemoglobin level in erythrocytes from mice subjected to γ rays from a 60Co source was detected using the methemoglobin kit. The dose range was from 0.5 to 8 Gy and the dose rate was 0.5 Gy/min. Results
The results demonstrate that methemoglobin level increases with increasing dose. The detection limit based on methemoglobin has a lower limit of dose estimation of about 1 Gy. The high levels of methemoglobin are maintained for at least 28 days, and the maximal increase of methemoglobin observed occurs at about 30 min after γ irradiation. The relationship between dosage and the increased methemoglobin level can be expressed by a linear quadratic equation of y = -8.75 x2 + 168.09 x + 32.66, with the correlation coefficient, r, equal to 0.96. The best suggested time for blood collection is up to 1 day after γ irradiation. The doses absorbed by mice as estimated from the use of the dose-response relationship were close to the blind doses of 1, 2, 4 and 8 Gy. Conclusion
Methemoglobin is a quick, simple, and precise biomarker for the early assessment of the absorbed dose in mice.Keywords:
Language:
English
Published:
International Journal of Radiation Research, Volume:13 Issue: 3, Jul 2015
Pages:
235 to 241
magiran.com/p1469635
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!