Antimicrobial effect of Fe2O3 nanoparticles functionalizing with imipenem on Pseudomonas aeruginosa producing Metallo β-lactamases

Multi-drug resistance strains of Pseudomonas aeruginosa which are particularly resistant to imipenem are considered as a new problem for treatment in the medical settings, especially in burnt units of the hospitals. This study was conducted to determine and evaluate the antimicrobial effect of ferrous oxide nanoparticles alone and functionalized with imipenem on Pseudomonas aeruginosa starins producing Metallo β-lactamases (MBL). A clinical sample of above noted bacterium resistant to commonly used antibiotics and concurrently resistant to imipenem antibiotic was diagnosed and isolated by disk diffusion method. The minimum inhibitory concentration (MIC) of bacteria was calculated in the presence of ferrous oxide nanoparticle. The antimicrobial effect of nanoparticles functionalized with antibiotic was also determined. Standard strain of P. aeruginosa ATCC: 27853 was used as control. The clinical sample was resistant to imipenem up to the concentration of 128 μg/ml. Similarly, MIC of the isolate for nanoparticle was 160 μg/ml. In course, it was observed that the combination of 16 μg of antibiotic with 80 μg/ml of Ferrous oxide nanoparticles were able to inhibit the growth of the isolate. Ferrous oxide nanoparticles can impair antibiotic resistance mechanisms of bacteria; moreover, it can make the imipenem resistant bacterium more susceptible to weaker concentrations of antibiotic. It also has its own antibacterial effect in certain concentrations